COMBINED IMMUNOCYTOCHEMISTRY AND ENZYME CYTOCHEMISTRY ON ULTRA-THIN CRYOSECTIONS - A NEW METHOD

被引：14

作者：

TAKIZAWA, T ^{[1
]}

ROBINSON, JM ^{[1
]}

机构：

[1] OHIO STATE UNIV,DEPT CELL BIOL NEUROBIOL & ANAT,4072 GRAVES HALL,333 W 10TH AVE,COLUMBUS,OH 43210

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1993年 / 41卷 / 11期

关键词：

IMMUNOCYTOCHEMISTRY; ENZYME CYTOCHEMISTRY; NEUTROPHILS; ALKALINE PHOSPHATASE; CERIUM; CRYOULTRAMICROTOMY;

D O I：

10.1177/41.11.8409371

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We combined immunocytochemistry and enzyme cytochemistry to localize two different proteins on the same ultra-thin cryosection. In this method the immunocytochemical localization is visualized with colloidal gold probes and the enzyme cytochemical detection is achieved with cerium as the capture agent. The immunocytochemistry is conducted first so that any potential adverse effects of the enzyme cytochemical procedure will not alter the antibody binding properties of the cryosections.

引用

页码：1635 / 1639

页数：5

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