SUPERANTIGENS ASSOCIATED WITH STAPHYLOCOCCAL AND STREPTOCOCCAL TOXIC SHOCK SYNDROME ARE POTENT INDUCERS OF TUMOR NECROSIS FACTOR-BETA SYNTHESIS

The role of tumor necrosis factor-alpha (TNFalpha) in the pathogenesis of severe bacterial infections has been studied extensively. However, the role of TNFbeta, a lymphokine with biologic activities similar to those of TNFalpha, has received little attention. Therefore, the purpose of this study was to examine the production of TNFbeta by peripheral blood mononuclear cells in response to lipopolysaccharide (LPS) and the superantigens staphylococcal toxic shock syndrome toxin 1 (TSST-1) and streptococcal pyrogenic exotoxin A (SPEA). Though LPS was a more potent inducer of TNFalpha than was TSST-1 or SPEA, TSST-1 and SPEA were both more potent inducers of TNFbeta. The superantigens TSST-1 and SPEA were more potent inducers of total TNF (TNFalpha and TNFbeta) than was LPS. These data suggest that the induction of TNFbeta synthesis may be a unique pathway by which superantigens associated with severe streptococcal and staphylococcal infections mediate shock and multiorgan failure characteristic of toxic shock syndrome.