ALTERATION OF HUMAN-ERYTHROCYTE PLASMA-MEMBRANES BY PERFRINGOLYSIN-O AS REVEALED BY FREEZE-FRACTURE ELECTRON-MICROSCOPY - STUDIES ON CLOSTRIDIUM-PERFRINGENS EXOTOXINS-V

被引:36
作者
MITSUI, K [1 ]
SEKIYA, T [1 ]
NOZAWA, Y [1 ]
HASE, J [1 ]
机构
[1] GIFU UNIV, SCH MED, DEPT BIOCHEM, GIFU, JAPAN
关键词
(Clostridium perfringens); Electron microscopy; Erythrocyte membrane; Freeze-fracture; Perfringolysin O; θ-Toxin;
D O I
10.1016/0005-2736(79)90007-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
When human erythrocyte membranes were treated with perfringolysin O (C. perfringens .theta.-toxin) and examined by EM after freeze-fracture, 2 ultrastructural alterations were observed in fracture faces of membrane. A random aggregation of intramembranous particles was seen in the fracture face of the protoplasmic half (PF face) of all membranes treated with the toxin, even if at a low concentration (40 hemolytic units/ml). The aggregation in the fracture face of the exoplasmic half (EF face) was observed only in membranes treated with a high concentration (3300 hemolytic units/ml) for 2 h. Round protrusions and ''cavities'' with 30 nm in diameter were visible in EF and PF faces of membranes treated with a high concentration, respectively. These structures were always protruded toward the cytoplasmic side but did not appear to form holes through the membrane. Ring and arc shaped structures with a dark center of 26 nm and a distinct border of 5 nm in width were observed when the toxin alone are negatively stained at a very high concentration (170,000 hemolytic units/ml). These structures were produced in the presence of cholesterol even if the toxin concentration was low.
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页码:68 / 75
页数:8
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