ASSAY OF ASCORBIC-ACID IN HUMAN CREVICULAR FLUID FROM CLINICALLY HEALTHY GINGIVAL SITES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：12

作者：

MEYLE, J ^{[1
]}

KAPITZA, K ^{[1
]}

机构：

[1] UNIV TUBINGEN, CTR DENT & MAXILLOFACIAL SURG, ORAL SURG & PERIODONT POLYCLIN, W-7400 TUBINGEN 1, GERMANY

来源：

ARCHIVES OF ORAL BIOLOGY | 1990年 / 35卷 / 04期

关键词：

ascorbic acid; gingival crevicular fluid;

D O I：

10.1016/0003-9969(90)90049-G

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The volume of fluid on filter paper strips was measured with a Periotron, eluted, and the ascorbic acid measured by chromatography. In preliminary experiments, pre-impregnation of the strips with citric acid increased the recovery of standard ascorbic acid from 37 to 89% and significantly reduced loss of water from the strips over a 3 min period. Samples of crevicular fluid were then collected from clinically healthy gingival sites of 21 healthy volunteers using pre-impregnated strips and assayed for ascorbic acid concentration, together with samples of blood plasma. The mean ascorbic acid concentration in gingival crevicular fluid (207.3 μmol/l; SD: ± 81.8) was significantly higher (p < 0.001) than the corresponding; plasma concentration (mean 72 μmol/l; SD: ± 23.3). © 1990.

引用

页码：319 / 323

页数：5

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