ACTIVE HYDROGEN BY CHEMICAL IONIZATION MASS-SPECTROMETRY

被引：59

作者：

LIN, YY

SMITH, LL

机构：

[1] Division of Biochemistry, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas

来源：

BIOMEDICAL MASS SPECTROMETRY | 1979年 / 6卷 / 01期

关键词：

D O I：

10.1002/bms.1200060104

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A means of determination of active hydrogen of hydroxyl, carboxyl, sulfhydryl, amino, amido and sulfonamido groups by chemical ionization mass spectrometry using ammonia and trideuterioammonia as reagent gases is described. The method is based on exchange of active hydrogen for deuterium occurring during the chemical ionization process using trideuterioammonia, with comparison of m/z for ammonium adduct [M+NH4]+ and trideuterioammonium adduct [M‐nH+nD+ND4]+, or of protonated molecular ion [M+H]+ and deuteronated molecular ion [M‐nH+nD+D]+ yielding the number of active hydrogens. Applications have been made to several classes of biologically important compounds. Copyright © 1979 Heyden & Son Ltd

引用

页码：15 / 18

页数：4

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