TYROSINE HYDROXYLASE-IMMUNOREACTIVE ELEMENTS IN THE DISTAL RETINA OF BUFO-MARINUS - A LIGHT AND ELECTRON-MICROSCOPIC STUDY

Tyrosine hydroxylase-immunoreactive elements in the distal retina of Bufo marinus were investigated using light and electron microscopic immunocytochemistry. At the light microscopic level, immunoreactive somas were seen in the proximal part of the inner nuclear layer, and immunoreactive processes projected both to the inner and outer plexiform layers. In some instances stained axon-like processes traveled from the inner plexiform layer, across the inner nuclear layer to the distal retina. Immunolabeled elements formed basket-like structures around the photoreceptor inner segments. At the ultrastructural level immunostained fibers were observed in close contact with the necks, lateral walls, bases and the outer surfaces of rod outer segments. Synaptic specializations were neither observed at rod contacts nor at other possible contact sites such as bipolar dendrites and horizontal cell somata and processes in the outer plexiform layer. In contrast, synaptic specializations between immunolabeled profiles and amacrine, bipolar and ganglion cells were regularly present in the inner plexiform layer. These findings suggest that a population of dopaminergic interplexiform cells is present in the Bufo retina and sends axon-like processes towards the distal retina. It is assumed that dopamine is probably released non-synaptically from the immunolabeled terminals in the distal retina influencing rods directly, by which the quality of photopic vision is enhanced in the anuran retina.