GENETIC SUPPRESSION ANALYSIS OF SIGMA(E)-INTERACTION WITH 3 PROMOTERS IN SPORULATING BACILLUS-SUBTILIS

被引：16

作者：

DIEDERICH, B ^{[1
]}

TATTI, KM ^{[1
]}

JONES, CH ^{[1
]}

BEALL, B ^{[1
]}

MORAN, CP ^{[1
]}

机构：

[1] EMORY UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL,ATLANTA,GA 30322

来源：

GENE | 1992年 / 121卷 / 01期

关键词：

RNA POLYMERASE; SIGMA-FACTOR; TRANSCRIPTION;

D O I：

10.1016/0378-1119(92)90162-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Genetic evidence suggests that the sigma (sigma) subunit of RNA polymerase determines the specificity of promoter utilization by making sequence-specific contacts with DNA. We examined the effects of two single amino acid(aa) substitutions in sigma(E) on the utilization of mutated derivatives of three different promoters in sporulating Bacillus subtilis. We found allele-specific suppression of mutations in all three promoters by each sigma(E) substitution in e. These results provide strong evidence that sigma(E) interacts with each of these promoters in vivo. Moreover, the specificity of suppression of the mutations by the aa substitutions in sigma(E) lead us to speculate that the Met124 of sigma(E) closely contacts two adjacent bp in the -10 region of the promoters.

引用

页码：63 / 69

页数：7

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