DETECTION OF FELINE IMMUNODEFICIENCY VIRUS BY A NESTED POLYMERASE CHAIN-REACTION

被引:19
作者
RIMSTAD, E
UELAND, K
机构
[1] NATL VET INST,POB 8156,N-0033 OSLO,NORWAY
[2] NORWEGIAN COLL VET MED,DEPT MICROBIOL & IMMUNOL,OSLO 1,NORWAY
关键词
FELINE IMMUNODEFICIENCY VIRUS; POLYMERASE CHAIN REACTION; VIRAL DIAGNOSIS;
D O I
10.1016/0166-0934(92)90055-I
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A specific and sensitive polymerase chain reaction (PCR) procedure for the detection of feline immunodeficiency virus (FIV) in peripheral blood mononuclear cells (PBMC) was developed. PBMC from both blood samples and cultures were digested by proteinase K in a lysis buffer, and after heat inactivation of the proteinase, the resultant material was used in a two step amplification protocol using nested sets of primers. Two independent amplifications, from the gag and pol genes respectively, were performed in each tube. The PCR was positive for six of 14 samples from FIV seropositive adult cats, while all 36 samples from seronegative cats were negative. In comparison with an antigen-capturing ELISA procedure, the PCR detected FIV infection in PBMC cultures on average two days earlier.
引用
收藏
页码:239 / 248
页数:10
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