CLONING AND CHARACTERIZATION OF A NEUROSPORA-CRASSA GENE REQUIRED FOR (1,3)BETA-GLUCAN SYNTHASE ACTIVITY AND CELL-WALL FORMATION

被引:28
作者
ENDERLIN, CS [1 ]
SELITRENNIKOFF, CP [1 ]
机构
[1] UNIV COLORADO,HLTH SCI CTR,DEPT CELLULAR & STRUCT BIOL,DENVER,CO 80262
关键词
GS-1; OSMOTIC; 1; GENE;
D O I
10.1073/pnas.91.20.9500
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The glucan synthase 1 gene (gs-1) is required for (1,3)beta-glucan synthase activity [E.C. 2.4.1.34; UDP glucose:1,3-beta-D-glucan 3-beta-D-glucosyltransferase] and for cell wall formation. The gs-1 gene was cloned by functional complementation of the cell-wall-less defect of the (1,3)beta-glucan synthase-deficient mutant, TM1, by using a genomic Neurospora crassa cosmid library. A 2568-nucleotide gs-1 cDNA sequence revealed a 532-amino acid open reading frame encoding a polypeptide of 59 kDa. The predicted gs-1 gene product has no obvious signal peptide cleavage sites or transmembrane domains. A gs-1 null mutant is defective for cell wall formation and (1,3)beta-glucan synthase activity. The predicted GS-1 protein is weakly homologous to a putative Saccharomyces cerevisiae transcriptional regulatory protein.
引用
收藏
页码:9500 / 9504
页数:5
相关论文
共 32 条
  • [1] GENERAL-METHOD FOR CLONING NEUROSPORA-CRASSA NUCLEAR GENES BY COMPLEMENTATION OF MUTANTS
    AKINS, RA
    LAMBOWITZ, AM
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (09) : 2272 - 2278
  • [2] PURIFICATION OF 1,3-BETA-GLUCAN SYNTHASE FROM NEUROSPORA-CRASSA BY PRODUCT ENTRAPMENT
    AWALD, P
    ZUGEL, M
    MONKS, C
    FROST, D
    SELITRENNIKOFF, CP
    [J]. EXPERIMENTAL MYCOLOGY, 1993, 17 (02): : 130 - 141
  • [3] GENBANK
    BENSON, D
    LIPMAN, DJ
    OSTELL, J
    [J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (13) : 2963 - 2965
  • [4] CHARACTERIZATION OF ASPERGILLUS-NIDULANS MUTANTS DEFICIENT IN CELL-WALL CHITIN OR GLUCAN
    BORGIA, PT
    DODGE, CL
    [J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (02) : 377 - 383
  • [5] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [6] BROWN JL, 1993, GENETICS, V133, P837
  • [7] TRANSFORMATION OF ASPERGILLUS-NIDULANS WITH THE HYGROMYCIN-RESISTANCE GENE, HPH
    CULLEN, D
    LEONG, SA
    WILSON, LJ
    HENNER, DJ
    [J]. GENE, 1987, 57 (01) : 21 - 26
  • [8] THE SCHIZOSACCHAROMYCES-POMBE CWG2+ GENE CODES FOR THE BETA-SUBUNIT OF A GERANYLGERANYLTRANSFERASE TYPE-I REQUIRED FOR BETA-GLUCAN SYNTHESIS
    DIAZ, M
    SANCHEZ, Y
    BENNETT, T
    SUN, CR
    GODOY, C
    TAMANOI, F
    DURAN, A
    PEREZ, P
    [J]. EMBO JOURNAL, 1993, 12 (13) : 5245 - 5254
  • [9] YEAST CALMODULIN AND A CONSERVED NUCLEAR-PROTEIN PARTICIPATE IN THE INVIVO BINDING OF A MATRIX ASSOCIATION REGION
    FISHEL, BR
    SPERRY, AO
    GARRARD, WT
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) : 5623 - 5627
  • [10] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING
    HENIKOFF, S
    [J]. GENE, 1984, 28 (03) : 351 - 359