TIME-RESOLVED CALCIUM MICRODOMAINS AND SYNAPTIC TRANSMISSION

被引：24

作者：

LLINAS, R ^{[1
]}

SUGIMORI, M ^{[1
]}

SILVER, RB ^{[1
]}

机构：

[1] MARINE BIOL LAB, WOODS HOLE, MA 02543 USA

来源：

JOURNAL OF PHYSIOLOGY-PARIS | 1995年 / 89卷 / 02期

关键词：

SQUID GIANT SYNAPSE; N-AEQUORIN-J; LIGHT EMISSION; IMAGING;

D O I：

10.1016/0928-4257(96)80554-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The time course for the calcium entry that triggers release was studied at the squid giant synapse by imaging light emission from n-aequorin-J intracellularly injected into the presynaptic terminal. The imaging utilized a video system capable of acquiring 4000 frames per second. The results indicate that the calcium entry triggered by action potentials reaches a peak within 200 microseconds and has an overall duration of close to 800 microseconds, closely matching the duration of the presynaptic calcium current determined by voltage clamp results under similar conditions.

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收藏

页码：77 / 81

页数：5

相关论文

共 22 条

[1] THE CALCIUM SIGNAL FOR TRANSMITTER SECRETION FROM PRESYNAPTIC NERVE-TERMINALS [J].

AUGUSTINE, GJ ;

ADLER, EM ;

CHARLTON, MP .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1991, 635 :365-381

[2] CALCIUM DOMAINS ASSOCIATED WITH INDIVIDUAL CHANNELS CAN ACCOUNT FOR ANOMALOUS VOLTAGE RELATIONS OF CA-DEPENDENT RESPONSES [J].

CHAD, JE ;

ECKERT, R .

BIOPHYSICAL JOURNAL, 1984, 45 (05) :993-999

[3]

COUTEAUX R, 1970, Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences Serie D Sciences Naturelles, V271, P2346

[4]

Heuser JE, 1977, HDB PHYSL, VI, P261

[5] A STUDY OF SYNAPTIC TRANSMISSION IN ABSENCE OF NERVE IMPULSES [J].

KATZ, B ;

MILEDI, R .

JOURNAL OF PHYSIOLOGY-LONDON, 1967, 192 (02) :407-+

[6] EFFECTS OF SYNAPSIN-I AND CALCIUM CALMODULIN-DEPENDENT PROTEIN KINASE-II ON SPONTANEOUS NEUROTRANSMITTER RELEASE IN THE SQUID GIANT SYNAPSE [J].

LIN, JW ;

SUGIMORI, M ;

LLINAS, RR ;

MCGUINNESS, TL ;

GREENGARD, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8257-8261

[7] TRANSMISSION BY PRE-SYNAPTIC SPIKE-LIKE DEPOLARIZATION IN THE SQUID GIANT SYNAPSE [J].

LLINAS, R ;

SUGIMORI, M ;

SIMON, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (07) :2415-2419

[8] BLOCKING AND ISOLATION OF A CALCIUM-CHANNEL FROM NEURONS IN MAMMALS AND CEPHALOPODS UTILIZING A TOXIN FRACTION (FTX) FROM FUNNEL-WEB SPIDER POISON [J].

LLINAS, R ;

SUGIMORI, M ;

LIN, JW ;

CHERKSEY, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) :1689-1693

[9] MICRODOMAINS OF HIGH CALCIUM-CONCENTRATION IN A PRESYNAPTIC TERMINAL [J].

LLINAS, R ;

SUGIMORI, M ;

SILVER, RB .

SCIENCE, 1992, 256 (5057) :677-679

[10] RELATIONSHIP BETWEEN PRESYNAPTIC CALCIUM CURRENT AND POSTSYNAPTIC POTENTIAL IN SQUID GIANT SYNAPSE [J].

LLINAS, R ;

STEINBERG, IZ ;

WALTON, K .

BIOPHYSICAL JOURNAL, 1981, 33 (03) :323-351