STUDIES ON 2 GLUTAMINASE SYSTEMS FROM RAT KIDNEY

被引：8

作者：

LAMAR, C

机构：

[1] The Department of Medicine, University of Wisconsin, School of Medicine, Madison, WI

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 151卷 / 01期

关键词：

D O I：

10.1016/0005-2744(68)90173-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme, glutamohydroxamase, that accomplishes the hydrolysis of γ-glutamohydroxamate and the deamidation of glutamine at similar rates has been described. This enzyme is predominantly present in rat kidney, is absent from liver, and is bound to the microsomal fraction of the cell. It is activated by SO42- and inhibited by HgCl2 and reduced glutathione in a similar manner as is glutaminase, which it resembles in several other ways as well. However, the failure of glutaminase to hydrolyze γ-glutamohydroxamate serves to distinguish the two enzymes. The products of the glutamohydroxamase reaction were shown to be glutamic acid and hydroxylamine and the reaction was found not to be reversible. © 1968.

引用

页码：188 / &

共 21 条

[1]

BOYER PD, 1960, ENZYMES ED, V4, P285

[2] The estimation of glutamine in the presence of asparagine. [J].