A RAPID ASSAY FOR 14C-LABELED HYDROXYLYSINE IN COLLAGEN AND RELATED MATERIALS

被引：40

作者：

BLUMENKRANTZ, N

PROCKOP, DJ

机构：

[1] Department of Medicine and Biochemistry, University of Pennsylvania, Philadelphia General Hospital, Philadelphia

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 30卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90130-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid technique for assaying 14C-labeled hydroxylysine in biological materials is reported. The assay depends on the periodate oxidation of 14C-hydroxylysine to give 14C-formaldehyde. The 14C-formaldehyde is reacted with dimedon and the resulting 14C-formaldemethone complex is extracted into toluene. The method should be applicable to studying the synthesis and metabolism of hydroxylysine in collagen and in related glycoproteins of tissues such as basement membranes. The method distinguishes between glycosylated and nonglycosylated 14C-hydroxylysine, and therefore it is applicable to studying the glycosylation of hydroxylysine in collagen and similar proteins. © 1969.

引用

页码：377 / +

页数：1

共 11 条

[1]

ARONSON RB, 1967, J BIOL CHEM, V242, P809

[2]

BUTLER WT, 1966, J BIOL CHEM, V241, P3882

[3]

CUNNINGHAM LW, 1967, J BIOL CHEM, V242, P2570

[4] ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM GLOMERULAR BASEMENT MEMBRANE [J].

KEFALIDES, NA .

BIOCHEMISTRY, 1968, 7 (09) :3103-+

[5] ISOLATION AND CHARACTERIZATION OF PEPTIDES DERIVED FROM ALPHA 1 CHAIN OF CHICK BONE COLLAGEN AFTER CYANOGEN BROMIDE CLEAVAGE [J].

MILLER, EJ ;

LANE, JM ;

PIEZ, KA .

BIOCHEMISTRY, 1969, 8 (01) :30-&

[6] RELATIONSHIP OF HYDROXYPROLINE EXCRETION IN URINE TO COLLAGEN METABOLISM - BIOCHEMISTRY AND CLINICAL APPLICATIONS [J].

PROCKOP, DJ ;

KIVIRIKK.KI .

ANNALS OF INTERNAL MEDICINE, 1967, 66 (06) :1243-+

[7]

PROCKOP DJ, 1968, TREATISE COLLAGEN

[8] SEQUENTIAL HYDROXYLATION OF LYSINE AND GLYCOSYLATION OF HYDROXYLYSINE DURING BIOSYNTHESIS OF COLLAGEN IN ISOLATED CARTILAGE [J].

ROSENBLOOM, J ;

BLUMENKRANTZ, N ;

PROCKOP, DJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 31 (05) :792-+

[9]

SPIRO RG, 1967, J BIOL CHEM, V242, P4813

[10]

Van Slyke DD, 1940, J BIOL CHEM, V133, P287

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