CORTICOSTEROIDOGENESIS AND CYTOCHROME LEVELS IN REGENERATING RAT ADRENAL

被引:11
作者
GALLANT, S
BROWNIE, AC
机构
[1] Departments of Pathology and Biochemistry, State University of New York at Buffalo, Buffalo
关键词
D O I
10.1016/0003-9861(69)90416-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homogenate preparations from the regenerating rat adrenal have reduced 11β-hydroxylating activity. To further examine this finding, mitochondria from control and regenerating rat adrenals have been isolated 3, 5, and 8 weeks following adrenal enucleation. The levels of cytochrome P-450 and the normal respiratory chain cytochromes were measured in addition to the ability of these mitochondrial preparations to carry out steroid hydroxylations. After 3 and 5 weeks of regeneration cytochrome P-450 levels were 55 and 78%, respectively, of control levels while after 8 weeks the levels were comparable in both groups. Similar results were obtained for microsomal cytochrome P-450. For all time periods studied, regenerating adrenal mitochondrial 11β-hydroxylase activity was low when malate or malate plus NADP were sources of reducing equivalents. With NADPH, however, corticosterone production was normal. These findings suggest that decreased NADPH generation may be the major factor involved in the reduced 11β-hydroxylating activity of the regenerating rat adrenal. © 1969.
引用
收藏
页码:441 / &
相关论文
共 36 条
[1]  
BAJUSZ E, 1967, METHODS ACHIEVEME ED, V2, P257
[2]   STEROID FRACTIONS FROM INCUBATED NORMAL AND REGENERATED ADRENAL GLANDS OF MALE AND FEMALE RATS [J].
BIRMINGHAM, MK ;
ROCHEFORT, G ;
TRAIKOV, H .
ENDOCRINOLOGY, 1965, 76 (05) :819-+
[3]  
BLOCH K, 1966, BIOLOGICAL CHEMIC ED, P153
[4]   THE INVITRO ENZYMIC HYDROXYLATION OF STEROID HORMONES .1. FACTORS INFLUENCING THE ENZYMIC 11-BETA-HYDROXYLATION OF 11-DEOXYCORTICOSTERONE [J].
BROWNIE, AC ;
GRANT, JK .
BIOCHEMICAL JOURNAL, 1954, 57 (02) :255-263
[5]   ADRENAL CYTOCHROME LEVELS CORTICOSTEROIDOGENESIS AND RESPIRATORY ACTIVITY IN RAT FOLLOWING METHYLANDROSTENEDIOL TREATMENT [J].
BROWNIE, AC ;
SKELTON, FR ;
GALLANT, S ;
NICHOLLS, P ;
ELLIOTT, WB .
LIFE SCIENCES PART 1 PHYSIOLOGY AND PHARMACOLOGY AND PART 2 BIOCHEMISTRY GENERAL AND MOLECULAR BIOLOGY, 1968, 7 (13P1) :765-&
[6]   METABOLISM OF PROGESTERONE-4-14C BY ADRENAL HOMOGENATES FROM RATS WITH ADRENAL-REGENERATION HYPERTENSION [J].
BROWNIE, AC ;
SKELTON, FR .
STEROIDS, 1965, 6 (01) :47-&
[7]  
BROWNIE AC, 1968, FUNCTIONS ADRENAL CO, V2, P691
[8]   SPECTROPHOTOMETRIC STUDIES OF PIGMENTS OF ADRENAL CORTEX MITOCHONDRIA [J].
CAMMER, W ;
ESTABROOK, RW .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1967, 122 (03) :735-+
[9]  
ESTABROOK RW, 1966, BIOLOGICAL CHEMICAL, P153
[10]  
FRIEDMAN M, 1949, P SOC EXP BIOL MED, V70, P670