CONTROL OF 3-PHOSPHOGLYCERATE REDUCTION IN ISOLATED-CHLOROPLASTS BY THE CONCENTRATIONS OF ATP, ADP AND 3-PHOSPHOGLYCERATE

被引：52

作者：

ROBINSON, SP ^{[1
]}

WALKER, DA ^{[1
]}

机构：

[1] UNIV SHEFFIELD,DEPT BOT,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 545卷 / 03期

关键词：

(Chloroplast); 3-Phosphoglycerate reduction; Oxygen evolution; Phosphoglycerate kinase;

D O I：

10.1016/0005-2728(79)90161-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Oxygen evolution by reconstituted chloroplasts with 3-phosphoglycerate as substrate was inhibited by ADP. This inhibition was overcome by increased concentrations of 3-phosphoglycerate or by the addition of excess ATP. 2. 2. The initial rate of 3-phosphoglycerate reduction by chloroplast stromal extracts, measured as 3-phosphoglycerate-dependent oxidation of NADPH, was also dependent on the concentrations of 3-phosphoglycerate, ADP and ATP. 3. 3. Within the range of concentrations of 3-phosphoglycerate, ADP and ATP expected to occur within intact chloroplasts, the rate of 3-phosphoglycerate (PGA) reduction by stromal extract was proportional to the ratio [ATP] [PGA]/[ADP]. 4. 4. These results are consistent with the notion that the substrates and products of 3-phosphoglycerate kinase control the rate of 3-phosphoglycerate reduction via a mass action effect. © 1979.

引用

页码：528 / 536

页数：9

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