INVIVO EXPRESSION OF RAT-LIVER C-ERBA-BETA-THYROID HORMONE RECEPTOR IN YEAST (SACCHAROMYCES-CEREVISIAE)

被引：13

作者：

LU, C ^{[1
]}

YANG, YF ^{[1
]}

OHASHI, H ^{[1
]}

WALFISH, PG ^{[1
]}

机构：

[1] UNIV TORONTO,MT SINAI HOSP,SAMUEL LUNENFELD RES INST,THYROID RES LAB,600 UNIV AVE,TORONTO M5G 1X5,ONTARIO,CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91367-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To study thyroid hormone receptor (TR), we developed an in vivo expression system in yeast by using a copper-responsive yeast metallothionein promoter and ubiquitinfusion protein technology. The cDNA encoding full-length rat liver TRβ was expressed under the control of copper. The [125I]T3 binding activities to yeast extracts were significantly correlated with the added copper sulfate into the medium. Partially purified TR from the transformed yeast had a high hormone binding affinity (Kd=0.34) for T3 and could bind thyroid hormone response element in gel retardation analysis. © 1990.

引用

页码：138 / 142

页数：5

共 23 条

[1]

APRILETTI JW, 1988, J BIOL CHEM, V263, P9409

[2] PRIMARY STRUCTURE AND EVOLUTION OF RAT GROWTH-HORMONE GENE [J].