MODE OF ACTION OF A NEW ANTI-TUMOR ANTIBIOTIC, SPORAMYCIN

被引：7

作者：

OKAMOTO, M ^{[1
]}

KOMIYAMA, K ^{[1
]}

TAKESHIMA, H ^{[1
]}

YAMAMOTO, H ^{[1
]}

UMEZAWA, I ^{[1
]}

机构：

[1] KITASATO INST, MINATO KU, TOKYO 108, JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1979年 / 32卷 / 04期

关键词：

D O I：

10.7164/antibiotics.32.386

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sporamycin, an antitumor antibiotic, primarily inhibited DNA synthesis, while RNA and protein synthesis were not significantly affected in [human cervical carcinoma] HeLa S3 cells. The antibiotic also caused strand scission of cellular DNA. The effects were not observed when the cells were incubated at 0.degree. C before washing and subsequently incubated at 37.degree. C. The Tm [melting point] of calf thymus DNA decreased when incubated with sporamycin in vitro. Sporamycin did not affect DNA synthesis in vitro catalyzed by partially purified DNA polymerases .alpha., .beta. and .gamma. derived from [mouse] Ehrlich ascites cells.

引用

页码：386 / 391

页数：6

共 15 条

[1]

ACUREA AD, 1978, P NATL ACAD SCI USA, V75, P3608

[2] CELLULAR DNA DAMAGE BY ANTI-TUMOR PROTEIN MACROMOMYCIN AND ITS RELATIONSHIP TO CELL-GROWTH INHIBITION [J].