HETEROGENEITY IN THE XENOPUS RIBOSOMAL TRANSCRIPTION FACTOR XUBF HAS A MOLECULAR-BASIS DISTINCT FROM THAT IN MAMMALS

被引:16
作者
BACHVAROV, D [1 ]
NORMANDEAU, M [1 ]
MOSS, T [1 ]
机构
[1] UNIV LAVAL,HOTEL DIEU QUEBEC,CTR RECH CANCEROL,11 COTE PALAIS,QUEBEC CITY G1R 2J6,QUEBEC,CANADA
关键词
RNA POLYMERASE-I; TRANSCRIPTION FACTOR; RIBOSOMAL; XUBF; XENOPUS-LAEVIS;
D O I
10.1016/0014-5793(91)81002-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Xenopus polymerase I transcription factor xUBF is an HMG-box protein which has been purified as two polypeptides of approximately 82 and 85 kDa. Recently a cDNA sequence predicted an xUBF protein (xUBF1) of 677 amino acids (79 kDa) containing five tandem HMG-boxes. Here a second and distinct xUBF cDNA has been isolated and characterised. This cDNA codes an xUBF protein (xUBF2) of 701 amino acids (82 kDa), having 93% homology with xUBF1 but containing an insertion of 22 amino acids between HMG-boxes 3 and 4. In vitro translation of synthetic mRNAs derived from the xUBF1 and 2 cDNAs was used to show that the electrophoretic mobility of the gene products accounted for the major xUBF molecular weight heterogeneity noted in vivo. It is also shown that the Xenopus laevis genome contains 3 or 4 distinct loci which hybridise with xUBF coding sequences, leaving open the possibility of yet further unrecognised heterogeneity in xUBF.
引用
收藏
页码:55 / 59
页数:5
相关论文
共 19 条
  • [1] THE RNA POLYMERASE-I TRANSCRIPTION FACTOR XUBF CONTAINS 5 TANDEMLY REPEATED HMG HOMOLOGY BOXES
    BACHVAROV, D
    MOSS, T
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (09) : 2331 - 2335
  • [2] MOLECULAR MECHANISMS GOVERNING SPECIES-SPECIFIC TRANSCRIPTION OF RIBOSOMAL-RNA
    BELL, SP
    PIKAARD, CS
    REEDER, RH
    TJIAN, R
    [J]. CELL, 1989, 59 (03) : 489 - 497
  • [3] FUNCTIONAL COOPERATIVITY BETWEEN TRANSCRIPTION FACTOR-UBF1 AND FACTOR-SL1 MEDIATES HUMAN RIBOSOMAL-RNA SYNTHESIS
    BELL, SP
    LEARNED, RM
    JANTZEN, HM
    TJIAN, R
    [J]. SCIENCE, 1988, 241 (4870) : 1192 - 1197
  • [4] ASSEMBLY OF ALTERNATIVE MULTIPROTEIN COMPLEXES DIRECTS RIBOSOMAL-RNA PROMOTER SELECTIVITY
    BELL, SP
    JANTZEN, HM
    TJIAN, R
    [J]. GENES & DEVELOPMENT, 1990, 4 (06) : 943 - 954
  • [5] A GENE-MAPPING TO THE SEX-DETERMINING REGION OF THE MOUSE Y-CHROMOSOME IS A MEMBER OF A NOVEL FAMILY OF EMBRYONICALLY EXPRESSED GENES
    GUBBAY, J
    COLLIGNON, J
    KOOPMAN, P
    CAPEL, B
    ECONOMOU, A
    MUNSTERBERG, A
    VIVIAN, N
    GOODFELLOW, P
    LOVELLBADGE, R
    [J]. NATURE, 1990, 346 (6281) : 245 - 250
  • [6] NUCLEOLAR TRANSCRIPTION FACTOR HUBF CONTAINS A DNA-BINDING MOTIF WITH HOMOLOGY TO HMG PROTEINS
    JANTZEN, HM
    ADMON, A
    BELL, SP
    TJIAN, R
    [J]. NATURE, 1990, 344 (6269) : 830 - 836
  • [7] KOLODRUBETZ D, 1990, J BIOL CHEM, V265, P3234
  • [8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
    LAEMMLI, UK
    [J]. NATURE, 1970, 227 (5259) : 680 - +
  • [9] NEILSON DA, 1986, NUCLEIC ACIDS RES, V14, P5936
  • [10] IDENTIFICATION OF 2 FORMS OF THE RNA POLYMERASE-I TRANSCRIPTION FACTOR UBF
    OMAHONY, DJ
    ROTHBLUM, LI
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (08) : 3180 - 3184