DETERMINATION OF TRANSFER-RNA NUCLEOTIDE RESIDUES DIRECTLY INTERACTING WITH PROTEINS IN THE POSTTRANSLOCATED AND PRETRANSLOCATED RIBOSOMAL COMPLEXES

被引：14

作者：

ABDURASHIDOVA, GG ^{[1
]}

TSVETKOVA, EA ^{[1
]}

BUDOWSKY, EI ^{[1
]}

机构：

[1] ND ZELINSKII ORGAN CHEM INST, MOSCOW 117913, USSR

来源：

FEBS LETTERS | 1990年 / 269卷 / 02期

关键词：

Escherichia coli ribosome; Localization of cross-linked tRNA residues; tRNA-binding site; tRNA-protein contacts; UV-induced tRNA-protein cross-links;

D O I：

10.1016/0014-5793(90)81202-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotide residues of E.coli tRNA interacting directly with proteins in pre- and posttranslocated ribosomal complexes have been identified by analysis of photo-induced tRNA-protein cross-links. A9, G 18, A26 and U59 residues of NAcPhePhe-tRNA, located in the Ab-site (pretranslocated complex) have been cross-linked with proteins S10, L27, S7 and L2 respectively. In deacylated tRNA, located in the Pb-site, residues C17, G44, C56 and U60 have been cross-linked with proteins L2, L5, L27 and S9 respectively. The G44-L5 cross-link disappeared after translocation (NAc-PhePhe-tRNA located in the Pt-site). © 1990.

引用

页码：398 / 401

页数：4

共 11 条

[1] STRUCTURAL CHARACTERISTICS AND CLASSIFICATION OF SOME TRANS RNA-BINDING SITES OF ELONGATING ESCHERICHIA-COLI RIBOSOME
ABDURASHIDOVA, GG
BASKAYEVA, IO
CHERNYI, AA
KAMINIR, LB
BUDOWSKY, EI
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 159 (01): : 103 - 109
[2] PREPARATION AND PROPERTIES OF MONOMERCURATED TRANSFER-RNA
ABDURASHIDOVA, GG
NARGISYAN, MG
BUDOWSKY, EI
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 136 (01): : 147 - 150
[3] NUCLEOTIDE RESIDUES OF TRANSFER-RNA, DIRECTLY INTERACTING WITH PROTEINS WITHIN THE COMPLEX OF THE 30-S SUBUNIT OF ESCHERICHIA-COLI RIBOSOME WITH POLY(U) AND NACPHE-TRANSFER RNAPHE
ABDURASHIDOVA, GG
TSVETKOVA, EA
BUDOWSKY, EI
[J]. FEBS LETTERS, 1989, 243 (02) : 299 - 302
[4] REACTIONS AT TERMINI OF TRANSFER-RNA WITH T4 RNA LIGASE
BRUCE, AG
UHLENBECK, OC
[J]. NUCLEIC ACIDS RESEARCH, 1978, 5 (10) : 3665 - 3677
[5] BUDOWSKY EI, 1989, PROG NUCLEIC ACID RE, V37, P1
[6] BEHAVIOUR OF ACETYLPHENYLALANYL SOLUBLE RIBONUCLEIC ACID IN POLYPHENYLALANINE SYNTHESIS
HAENNI, AL
CHAPEVILLE, F
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 114 (01) : 135 - +
[7] RIBOSOMAL PROTEINS .7. 2-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS FOR FINGERPRINTING OF RIBOSOMAL PROTEINS
KALTSCHM.E
WITTMANN, HG
[J]. ANALYTICAL BIOCHEMISTRY, 1970, 36 (02) : 401 - &
[8] MECHANISM OF CODON-ANTICODON INTERACTION IN RIBOSOMES - QUANTITATIVE STUDY OF CODON-DEPENDENT BINDING OF TRANSFER-RNA TO 30-S RIBOSOMAL-SUBUNITS OF ESCHERICHIA-COLI
KIRILLOV, SV
MAKHNO, VI
SEMENKOV, YP
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 89 (01): : 297 - 304
[9] RICH A, 1974, RIBOSOMES, P871
[10] KINETIC ASPECTS OF TETRACYCLINE ACTION ON THE ACCEPTOR (A) SITE OF ESCHERICHIA-COLI RIBOSOMES
SEMENKOV, YP
MAKAROV, EM
MAKHNO, VI
KIRILLOV, SV
[J]. FEBS LETTERS, 1982, 144 (01) : 125 - 129

← 1 2 →