INFLUENCE OF THE HOST-CELL ON PROTEINS SYNTHESIZED BY DIFFERENT STRAINS OF INFLUENZA-VIRUS

The synthesis of influenza virus proteins has been studied quantitatively in nine different virus-cell systems, using three strains and three cell lines. The systems were remarkable for their variation. The only common properties to emerge were that NS1 and NP were always synthesized earlier than M and HA0 and secondly, with the exception of the P proteins, proteins were synthesized in amounts proportional to their time of appearance, i.e., the first protein to appear was present in the greatest amount. Otherwise variations were found in the order of appearance of newly synthesized proteins, in the relative amounts synthesized, and in the total synthesized. These variations were unrelated to virus strain or cell type, to the yield of infectious virus, or to its infectivity: HA ratio, and to the shut-off of host protein synthesis. We conclude that the pattern of protein synthesis in a particular virus-cell system is unique. © 1979.