MESSENGER-RNA VARIANTS ENCODING MULTIPLE FORMS OF THE HIGH-AFFINITY IGE RECEPTOR ALPHA SUBUNIT IN TRANSFORMED AND NONTRANSFORMED MAST-CELLS

被引：8

作者：

ROBERTSON, MW ^{[1
]}

MEHL, VS ^{[1
]}

RICHARDS, ML ^{[1
]}

LIU, FT ^{[1
]}

机构：

[1] MED BIOL INST,LA JOLLA,CA

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY | 1991年 / 96卷 / 04期

关键词：

D O I：

10.1159/000235511

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Multiple mRNA species encoding several predicted forms of the high-affinity IgE receptor alpha-subunit (Fc-epsilon-RI-alpha) have been previously characterized from rat basophilic leukemia cells. Using the polymerase chain reaction procedure, we have extended these findings to show that one Fc-epsilon-RI-alpha mRNA variant, characterized by a 163-bp deletion within the coding sequence, exists in normal rat connective tissue mast cells as well as in both transformed and non transformed murine mast cell lines. In addition, a partial murine Fc-epsilon-RI-alpha genomic clone, spanning the internal-deletion sequence, has been identified, and from analysis of this sequence a mechanism of alternative pre-mRNA splicing is proposed. Finally, mRNA variants have been translated in a cell-free system and the protein products partially characterized.

引用

页码：289 / 295

页数：7

共 25 条

[1] GENERATION OF PROTEIN ISOFORM DIVERSITY BY ALTERNATIVE SPLICING - MECHANISTIC AND BIOLOGICAL IMPLICATIONS [J].