CA2+ CURRENTS OF FAST BLUE-LABELED SUPERIOR CERVICAL-GANGLION NEURONS

被引:8
作者
CHEN, C [1 ]
SCHOFIELD, GG [1 ]
机构
[1] TULANE UNIV,SCH MED,DEPT PHYSIOL,1430 TULANE AVE,NEW ORLEANS,LA 70112
关键词
SUPERIOR CERVICAL GANGLION; SYMPATHETIC NEURON; FLUORESCENT DYE; ULTRAVIOLET; VOLTAGE-CLAMP; ION CHANNEL; CALCIUM CURRENT;
D O I
10.1016/0165-0270(92)90044-E
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To investigated if Ca2+ currents of acutely isolated superior cervical ganglion (SCG) neurons are altered after dye labeling or ultraviolet (UV) exposure, SCG neurons were labeled by multiple injections of 2% fast blue (FB) (5-mul total) into the submaxillary gland. Ca2+ currents of both labeled and unlabeled neurons were significantly depressed by 2-min exposure to UV, compared to labeled and unlabeled neurons not exposed to UV. The I-V curve of labeled neurons was shifted hyperpolarized after UV exposure. Tail-current activation curves of both labeled and unlabeled neurons were fitted to a double Boltzmann equation. In labeled neurons, 2-min exposure to UV produced a significant reduction of the amplitude of the more depolarized component as well as a hyperpolarizing shift of the half activation potential of both components of the activation curve. However, the short time (< 10 s) required to identify a target-specific neuron had no significant effect on the biophysical properties of Ca2+ currents of FB-labeled SCG neurons. Thus, use of this label in combination with the patch-clamp technique comprises a powerful approach to study membrane currents of target-specific neurons.
引用
收藏
页码:63 / 69
页数:7
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