GENERATION OF MATRIX-DEGRADING PROTEOLYTIC SYSTEM FROM FIBRONECTIN BY CATHEPSIN-B, CATHEPSIN-G, CATHEPSIN-H AND CATHEPSIN-L

被引：17

作者：

BLONDEAU, X

VIDMAR, SL

EMOD, I

PAGANO, M

TURK, V

KEILDLOUHA, V

机构：

[1] INST PASTEUR, CHIM PROT LAB, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCE

[2] BROUSSAIS HOTEL DIEU, FAC MED, BIOCHIM LAB, F-75270 PARIS 06, FRANCE

[3] J STEFAN INST, DEPT BIOCHEM, LJUBLJANA 61000, SLOVENIA

来源：

BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1993年 / 374卷 / 08期

关键词：

FIBRONECTIN; CATHEPSIN-B; CATHEPSIN-G; CATHEPSIN-H; CATHEPSIN-L; FIBRONECTINASE; FN-TYPE-IV COLLAGENASE; MATRIX-DEGRADING ACTIVITY;

D O I：

10.1515/bchm3.1993.374.7-12.651

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By their endoproteinase activities, cathepsins B, G, H and L can generate matrix-degrading proteolytic system from fibronectin. All four cathepsins studied cleaved fibronectin in fragments that were either proteolytically active or activated after incubation at pH 7.4 and in the presence of Ca2+. The highest enhancement of the matrix protein-degrading activity was observed after a gelatin-affinity chromatography of each digest. These results suggest that the effect of cathepsins at physiological pH in vivo may be enhanced by the activation of a matrix-degrading proteolytic system from fibronectin.

引用

页码：651 / 656

页数：6

共 29 条

[1] BARRETT AJ, 1981, METHOD ENZYMOL, V80, P535
[2] BRZIN J, 1983, H-S Z PHYSIOL CHEM, V364, P1475, DOI 10.1515/bchm2.1983.364.2.1475
[3] CATHEPSIN-B1 - LYSOSOMAL ENZYME THAT DEGRADES NATIVE COLLAGEN
BURLEIGH, MC
BARRETT, AJ
LAZARUS, GS
[J]. BIOCHEMICAL JOURNAL, 1974, 137 (02) : 387 - +
[4] DEPETRO G, 1981, P NATL ACAD SCI-BIOL, V78, P4965
[5] DUNN AD, 1991, J BIOL CHEM, V266, P20198
[6] POTENTIAL PROTEOLYTIC ACTIVITY OF FIBRONECTIN - FIBRONECTIN LAMINASE AND ITS SUBSTRATE-SPECIFICITY
EMOD, I
LAFAYE, P
PLANCHENAULT, T
VIDMAR, SL
IMHOFF, JM
KEILDLOUHA, V
[J]. BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1990, 371 (02): : 129 - 135
[7] Gottesman M., 1990, SEMIN CANCER BIOL, V1, P97
[8] GUINEC N, 1990, BIOL CHEM H-S, V371, P239
[9] HEDMAN K, 1989, FIBRONECTIN, P123
[10] JOHANSSON S, 1986, J BIOL CHEM, V261, P4363

← 1 2 3 →