HEMOSTATIC PROPERTIES OF THE SV40 TRANSFECTED HUMAN MICROVASCULAR ENDOTHELIAL-CELL LINE (HMEC-1) - A REPRESENTATIVE IN-VITRO MODEL FOR MICROVASCULAR ENDOTHELIUM

被引：30

作者：

RIBEIRO, MJA

PHILLIPS, DJ

BENSON, JM

EVATT, BL

ADES, EW

HOOPER, WC

机构：

[1] US DEPT HHS,PUBL HLTH SERV,CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV HIV AIDS,ATLANTA,GA 30333

[2] EMORY UNIV HOSP,DEPT MED,DIV HEMATOL ONCOL,ATLANTA,GA 30322

来源：

THROMBOSIS RESEARCH | 1995年 / 79卷 / 02期

关键词：

MICROVASCULAR ENDOTHELIAL CELLS; TISSUE FACTOR; TISSUE PLASMINOGEN ACTIVATOR; PLASMINOGEN ACTIVATOR INHIBITOR-1;

D O I：

10.1016/0049-3848(95)00101-V

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

HMEC-1 is a SV-40T transfected human microvascular endothelial cell line that constitutively expresses RNA transcripts for plasminogen activator inhibitor 1 (PAI-1), tissue-type plasminogen activator (t-PA), protein S (PS), von Willebrand factor (vWF), and thrombomodulin. Tissue factor (TF) can be induced in response to stimulation with tumor necrosis factor alpha (TNF-alpha), interleukin-1 alpha (IL-1 alpha) and phorbol 12-myristate 13-acetate (PMA) Proteins corresponding to PAI-1, t-PA protein S and VWF genes were constitutively released in the culture supernatant. This cell line is a model that will be useful to investigate coagulation/fibrinolytic properties of microvascular endothelium.

引用

页码：153 / 161

页数：9

共 24 条

[1]

Swerlick, Lee, Wick, Lawley, Human dermal microvascular endothelial cell but not human umbilical vein endothelial cells express CD36 in vivo and in vitro, Journal of Immunology, 148, pp. 78-83, (1992)

[2]

Jaffe, Nachman, Becker, Minick, Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria, Journal of Clinical Investigation, 52, pp. 2745-2756, (1973)

[3]

Folkman, Haudenschild, Zetter, Long term culture of capillary endothelial cells, Proc. Natl. Acad. Sci. USA, 76, pp. 5217-5222, (1979)

[4]

Lassale, Lagrou, Delnete, Sanceau, Coll, Torpier, Wietzerbin, Stehelin, Tonnel, Capron, Human endothelial cells transfected by SV-40 T antigens: characterization and potential use as a source of normal endothelial factors, Eur. J. Immunol., 22, pp. 425-431, (1992)

[5]

Fickling, Tooze, Whitley, Characterization of human umbilical vein endothelial cell lines produced by transfection with the early region of SV40, Experimental Cell Research, 201, pp. 517-521, (1992)

[6]

Gimbrone, Fareed, Transformation of cultured human vascular endothelium by SV40 DNA, Cell, 9, pp. 685-693, (1976)

[7]

Ide, Minamishima, Eizuru, Okada, Sakihama, Katsuki, “Transformation” of human endothelial cells by SV40 virions, Microbiol. Immunol., 32, pp. 45-55, (1988)

[8]

Ades, Candal, Swerlick, George, Summers, Bosse, Lawley, HMEC-1: Establishment of an immortalized human microvascular endothelial cell line, J. Invest. Dermatol., 99, pp. 683-690, (1992)

[9]

Lal, Rudolph, Buckner, Pardi, Hooper, Infection with human T-lymphotropic viruses leads to constitutive expression of leukemia inhibitory factor and interleukin-6, Blood, 81, (1993)

[10]

Mullis, Faloona, Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction, Methods Enzymol, 155, pp. 335-350, (1987)

← 1 2 3 →