THE STABILIZATION OF A HUMAN-IGM MONOCLONAL-ANTIBODY WITH POLY(VINYLPYRROLIDONE)

被引:49
作者
GOMBOTZ, WR
PANKEY, SC
PHAN, D
DRAGER, R
DONALDSON, K
ANTONSEN, KP
HOFFMAN, AS
RAFF, HV
机构
[1] BRISTOL MYERS SQUIBB,PHARMACEUT RES INST,BIOL PROC RES,SEATTLE,WA 98121
[2] MILES INC,CUTTER BIOL,BERKELEY,CA 94701
[3] UNIV WASHINGTON,CTR BIOENGN FL 20,SEATTLE,WA 98195
关键词
PROTEIN STABILITY; PROTEIN AGGREGATION; MONOCLONAL ANTIBODIES; VOLUME EXCLUSION; POLY(VINYLPYRROLIDONE);
D O I
10.1023/A:1018903624373
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An IgM anti-group B Streptococcus monoclonal antibody (4B9) was found to undergo irreversible heat-induced aggregation at 50 degrees C. A variety of excipients was tested for their ability to inhibit antibody aggregation. The amount of 4B9 aggregation, which was determined by analysis on a size-exclusion HPLC, was significantly reduced in the presence of low concentrations [between 0.1 and 1.0% (w/v)] of poly(vinylpyrrolidone) (PVP) molecules ranging in molecular weight from 10 to 40 kDa. When the PVP concentration was greater than 1.05%, antibody aggregation was enhanced, and with the highest molecular weight PVP, antibody precipitation occurred. HPLC was used to show that more PVP was associated with the 4B9 at 50 degrees C than at 25 degrees C. Differential scanning calorimetry revealed that PVP concentrations greater than 2.0% decreased the antibody thermal transition temperature. Enzyme-linked immunosorbent assays were used to assess the effects of PVP on the antigen binding capacity of 4B9 and on 4B9 quantitation. At 4 degrees C, PVP solutions of up to 5.0% had no effect on either 4B9 quantitation or antigen binding. At 50 degrees C, however, less 4B9 was detected in the 5.0% PVP solution. The heat stabilization of the 4B9 antibody by low concentrations of PVP can be explained by a weak binding of PVP to the native protein. The PVP may sterically interfere with protein-protein interactions, thus reducing aggregation. Higher concentrations of PVP lead to protein aggregation and precipitation, probably by a volume-exclusion mechanism. Low concentrations of less than 1.0% PVP can be used to stabilize proteins against heat-induced aggregation, but care should be exercised, since even slightly higher concentrations of PVP can also lead to protein destabilization.
引用
收藏
页码:624 / 632
页数:9
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