A method was developed to study the grapevine dieback caused by Eutypa lata in the greenhouse. Plugs of mycelium and PDA medium were inserted into holes made 4 cm below the buds of unrooted cuttings. Shoots arising from inoculated cuttings exhibited typical foliar symptoms of Eutypa dieback as early as the F stage. Different isolates of E lata displayed considerable variation in the incidence of symptoms. All the isolates could colonize the cuttings but some of them did not induce foliar symptoms. All the inoculated cultivars presented the symptoms of the disease and varied in resistance.