USE OF LIQUID SCINTILLATION SPECTROMETER FOR DETERMINING ADENOSINE TRIPHOSPHATE BY LUCIFERASE ENZYME

被引：954

作者：

STANLEY, PE

WILLIAMS, SG

机构：

[1] Department of Agricultural Biochemistry, Waite Agricultural Research Institute, The University of Adelaide, SA

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90323-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, economical method is described for determining ATP over the range 10-9 and 10-12 mole, by the firefly luciferin-luciferase system, using any liquid scintillation spectrometer of suitable performance. This procedure has been employed to determine ATP in perchloric acid extracts of biological material. A critical appraisal is made of the factors affecting the liquid scintillation spectrometer for this assay. © 1969.

引用

页码：381 / &

共 22 条

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