PRIMARY STRUCTURE OF SORGHUM MALATE-DEHYDROGENASE (NADP) DEDUCED FROM CDNA SEQUENCE - HOMOLOGY WITH MALATE-DEHYDROGENASE (NAD)

被引：52

作者：

CRETIN, C ^{[1
]}

LUCHETTA, P ^{[1
]}

JOLY, C ^{[1
]}

DECOTTIGNIES, P ^{[1
]}

LEPINIEC, L ^{[1
]}

GADAL, P ^{[1
]}

SALLANTIN, M ^{[1
]}

HUET, JC ^{[1
]}

PERNOLLET, JC ^{[1
]}

机构：

[1] INRA, DEPT PHYSIOL & BIOCHIM VEGETALES, ETUD PROT LAB, F-78000 VERSAILLES, FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 192卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb19227.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Malate dehydrogenase (NADP) (NADP‐MDH) is an important enzyme of the photosynthetic CO2 fixation pathway of C4 plants. We have isolated two clones from a sorghum λgt11 cDNA library (CM3, 932 bp, and CM7, 1441 bp). Nucleotide sequence analysis of the cDNAs CM3 and CM7 showed the existence of two NADP‐MDH mRNA species encoding different enzyme subunits. Microsequencing of the N‐terminus of the mature protein indicated that a specific cleavage of 13 amino acids occurred during the purification steps of the enzyme. The full‐length cDNA CM7 contains a large open reading frame encoding an NH2‐terminal transit peptide of 40 amino acids and a mature protein of 389 amino acids (42.207 kDa). Alignment of the NADP‐MDH sequence with those of several malate dehydrogenases revealed some similarities with NAD‐MDHs. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：299 / 303

页数：5

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