CLONING AND CHARACTERIZATION OF A GENE ENCODING A NEW THERMOSTABLE HEMOLYSIN FROM VIBRIO-PARAHAEMOLYTICUS

被引:21
作者
TANIGUCHI, H
KUBOMURA, S
HIRANO, H
MIZUE, K
OGAWA, M
MIZUGUCHI, Y
机构
[1] UNIV ENVIRONM & OCCUPAT HLTH,SCH MED,DEPT SURG,KITAKYUSHU,FUKUOKA 807,JAPAN
[2] UNIV ENVIRONM & OCCUPAT HLTH,SCH MED,DEPT BIOCHEM,KITAKYUSHU,FUKUOKA 807,JAPAN
关键词
DNA-cloning; Hemolysin; a new; Sequencing; Vibrio parahaemolyticus;
D O I
10.1016/0378-1097(90)90020-Q
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A new thermostable hemolsin (σ-VPH) gene was cloned from a Kanagawa-negative Vibrio parahaemolyticus strain into vector pBR322 in Escherichia coli K12. The nucleotide and amino acid sequences had np homology with those of the thermostable direct hemolysin (TDH) which causes the Kanagawa phenomenon, and of the thermolabile hemolysin (TLH) of V. parahaemolyticus. The gene was present in all V. parahaemolyticus strains tested and also in one strain of V. damsela. © 1990.
引用
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页码:339 / 346
页数:8
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