HIGH-SPEED SEPARATIONS OF DNA SEQUENCING REACTIONS BY CAPILLARY ELECTROPHORESIS

被引：230

作者：

DROSSMAN, H ^{[1
]}

LUCKEY, JA ^{[1
]}

KOSTICHKA, AJ ^{[1
]}

DCUNHA, J ^{[1
]}

SMITH, LM ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 09期

关键词：

D O I：

10.1021/ac00208a003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorescently labeled DNA fragments generated In enzymatic sequencing reactions are rapidly separated by capillary gel electrophoresis and detected at attomole levels within the gel-filled capillary. The application of this technology to automated DNA sequence analysis may permit the development of a second generation automated sequencer capable of efficient and cost-effective sequence analysis on the genomic scale. © 1990 American Chemical Society. © 1990, American Chemical Society. All rights reserved.

引用

页码：900 / 903

页数：4

共 19 条

[1] BENTE PF, 1988, Patent No. 272925
[2] BENTE PF, 1836, Patent No. 946568
[3] SUBATTOMOLE AMINO-ACID ANALYSIS BY CAPILLARY ZONE ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE
CHENG, YF
DOVICHI, NJ
[J]. SCIENCE, 1988, 242 (4878) : 562 - 564
[4] RAPID SEPARATION AND PURIFICATION OF OLIGONUCLEOTIDES BY HIGH-PERFORMANCE CAPILLARY GEL-ELECTROPHORESIS
COHEN, AS
NAJARIAN, DR
PAULUS, A
GUTTMAN, A
SMITH, JA
KARGER, BL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) : 9660 - 9663
[5] COMPTON SW, 1988, BIOTECHNIQUES, V6, P432
[6] CONNELL C, 1987, BIOTECHNIQUES, V5, P342
[7] CAPILLARY ELECTROPHORESIS
EWING, AG
WALLINGFORD, RA
OLEFIROWICZ, TM
[J]. ANALYTICAL CHEMISTRY, 1989, 61 (04) : A292 - +
[8] HIGH-PERFORMANCE ELECTROPHORESIS - THE ELECTROPHORETIC COUNTERPART OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
HJERTEN, S
[J]. JOURNAL OF CHROMATOGRAPHY, 1983, 270 (NOV): : 1 - 6
[9] HOOD L E, 1987, Genomics, V1, P201, DOI 10.1016/0888-7543(87)90046-2
[10] BIAS IN QUANTITATIVE CAPILLARY ZONE ELECTROPHORESIS CAUSED BY ELECTROKINETIC SAMPLE INJECTION
HUANG, XH
GORDON, MJ
ZARE, RN
[J]. ANALYTICAL CHEMISTRY, 1988, 60 (04) : 375 - 377

← 1 2 →