EYE MUSCLE MEMBRANE REACTIVE ANTIBODIES ARE NOT DETECTED IN THE SERUM OR IMMUNOGLOBULIN FRACTION OF PATIENTS WITH THYROID-ASSOCIATED OPHTHALMOPATHY USING AN ELISA AND CRUDE MEMBRANES

被引：16

作者：

KAPUSTA, M ^{[1
]}

SALVI, M ^{[1
]}

TRILLER, H ^{[1
]}

GARDINI, E ^{[1
]}

BERNARD, N ^{[1
]}

WALL, JR ^{[1
]}

机构：

[1] MONTREAL GEN HOSP,RES INST,THYROID RES UNIT,1650 CEDAR AVE,MONTREAL H3G 1A4,QUEBEC,CANADA

来源：

AUTOIMMUNITY | 1990年 / 7卷 / 01期

基金：

美国国家卫生研究院; 英国医学研究理事会;

关键词：

64kDa antigen; Enzyme-linked immunosorbent assay (ELISA); Eye muscle antibodies; Ophthalmopathy; SDS-PAGE;

D O I：

10.3109/08916939009041048

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We tested sera and purified immunoglobulin (Ig) fractions from patients with autoimmune thyroid disorders (AITD), with and without ophthalmopathy, and normal subjects, for the presence of antibodies reactive with eye muscle membrane antigens in an optimized enzyme-linked immunosorbent assay (ELISA). We found no correlation between ELISA results and the presence or severity of ophthalmopathy in patients with AITD for either serum or Ig, and there were no significant differences between the mean values (± SE) for the three groups (AITD with ophthalmopathy, AITD without ophthalmopathy and normals) for either serum or Ig. In contrast Ig from 8 of 19 (45% patients with thyroid-associated ophthalmopathy reacted with a 64 kDa eye muscle membrane antigen in SDS-polyacrylamide gel elec-tropohoresis and Western blotting, while tests were positive in only one of the 8 patients with AITD without eye disease and in none of the 8 normal subjects. The presence of antibodies to a 64kDa antigen in immunoblotting did not correlate with the levels of antibodies measured in ELISA. We conclude that the ELISA, incorporating crude membrane fractions as antigen, is not useful as a clinical test for eye muscle autoantibodies. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：33 / 40

页数：8

共 15 条

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[2]

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