AMPLIFICATION AND ANALYSIS OF HUMAN DNA PRESENT IN MOSQUITO BLOODMEALS

被引：55

作者：

COULSON, RMR ^{[1
]}

CURTIS, CF ^{[1
]}

READY, PD ^{[1
]}

HILL, N ^{[1
]}

SMITH, DF ^{[1
]}

机构：

[1] IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,LONDON SW7 2AZ,ENGLAND

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 1990年 / 4卷 / 04期

关键词：

Anopheles gambiae; bloodmeal identification; DNA fingerprinting; mosquitoes; polymerase chain reaction;

D O I：

10.1111/j.1365-2915.1990.tb00452.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Abstract. DNA fingerprinting should permit the identification of individual human hosts of haematophagous arthropods, providing epidemi‐ologically useful information, for example, the biting rates on different people and the impact of insecticide‐impregnated bednets. Investigations reported here demonstrate that it is possible to extract, amplify and fingerprint human DNA from the bloodmeals of individual female Anopheles gambiae mosquitoes kept at 24oC for up to 10–15 h post‐ingestion. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：357 / 366

页数：10

共 17 条

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