INVESTIGATION OF COPPER-PTSM AS A PET TRACER FOR TUMOR BLOOD-FLOW

被引:36
作者
MATHIAS, CJ
WELCH, MJ
PERRY, DJ
MCGUIRE, AH
ZHU, X
CONNETT, JM
GREEN, MA
机构
[1] WASHINGTON UNIV,SCH MED,EDWARD MALLINCKRODT INST RADIOL,510 S KINGSHIGHWAY BLVD,BOX 8131,ST LOUIS,MO 63110
[2] WASHINGTON UNIV,SCH MED,DEPT SURG,ST LOUIS,MO 63110
[3] PURDUE UNIV,DEPT PHARM,W LAFAYETTE,IN 47907
来源
NUCLEAR MEDICINE AND BIOLOGY | 1991年 / 18卷 / 07期
关键词
D O I
10.1016/0883-2897(91)90022-D
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Copper-PTSM has been shown in previous studies to act as a fluid microsphere and to be useful in quantitating blood flow in brain, myocardium, and kidneys. In this study we have evaluated this agent as a PET tumor blood flow agent. Cu-64- or Cu-67-labeled Cu-PTSM was administered (i.v.) to Golden Syrian hamsters with colorectal carcinoma cell implants (GW39). One minute prior to sacrifice (10-60 min after Cu-PTSM was administered) I-125-iodoantipyrine (I-125-IAP), an agent known to measure tumor blood flow, was administered intravenously by a 3-stage, 1 min ramp infusion. Following sacrifice, samples of tumor and brain were removed (within 40 s) and the tumor and brain levels of Cu-PTSM and iodoantipyrine determined. Since the brain uptake of both Cu-PTSM and IAP is perfusion rate limited, the brain was used as a reference organ to normalize tumor levels of the two tracers. The plot of Cu-PTSM versus I-125-IAP tumor/brain ratios showed a good linear correlation (r value of 0.97), suggesting that Cu-PTSM could be used to quantify tumor blood flow. Since the mechanism of Cu-PTSM trapping is likely to be due to glutathione levels in the tissue, and because tumor tissue glutathione levels might vary, the temporal uptake of Cu-PTSM was investigated by PET imaging both the tumor-bearing hamsters and approximately 300 g Copenhagen rats bearing R3227 prostate tumors. The tumors were clearly visualized and the retained copper radioactivity in the tumor was constant over the 30 min imaging period.
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收藏
页码:807 / 811
页数:5
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