ADDITIVE INCREASE IN KIDNEY INSULIN-LIKE GROWTH FACTOR-I AND INITIAL RENAL ENLARGEMENT IN UNINEPHRECTOMIZED-DIABETIC RATS

被引:25
作者
FLYVBJERG, A
FRYSTYK, J
MARSHALL, SM
机构
[1] Second University Clinic of, Internal Medicine, Aarhus Kommunehospital
关键词
hypertrophy; insulin-like growth factor I; kidney; rat; streptozotocin diabetes; uninephrectomy;
D O I
10.1055/s-2007-1004961
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The initial renal hypertrophy in experimental diabetes and in response to uninephrectomy is associated with renal accumulation of insulin-like growth factor I (IGF-I). Since the combination of diabetes and nephrectomy almost doubles the initial renal growth rate the aim of the present study was to investigate the kidney IGF-I levels in the combined situation in uninephrectomized diabetic rats. Three experimental groups were exposed to either unilateral nephrectomy, streptozotocin-diabetes or both conditions and for four days animals from each group were taken out for investigation. After 4 days the wet kidney weight increased from baseline by 31% (from 661 ± 16 mg (SEM) to 866 ± 27 mg) (P < 0.01) in the uninephrectomized group, 32% (to 872 ± 18 mg) (P < 0.01) in the diabetic group and 46% (to 962 ± 27 mg) (P < 0.01) in the uninephrectomized-diabetic group. Kidney IGF-I concentrations were analyzed by radioimmunoassay and the increase from baseline on day 2 was 74% (from 262 ± 12 ng/g (SEM) to 456 ± 21 ng/g) (P < 0.01) in the uninephrectomized group, 58% (to 414 ± 18 ng/g) (P < 0.01) in the diabetic group and 176% (to 722 ± 56 ng/g) (P < 0.01) in the combined group. Thereafter a decline in kidney IGF-I occurred in all groups, being normal at day 4 for the diabetic group, but still significantly higher in the uninephrectomized and uninephrectomized-diabetic groups compared to controls (P < 0.05%). These results demonstrate that the combination of uninephrectomy and diabetes induces an additive enhancement of kidney IGF-I accumulation and of the renal growth response compared to the isolated effect of the two conditions, giving further evidence for a causal role of IGF-I in diabetic and post-nephrectomy renal growth.
引用
收藏
页码:516 / 520
页数:5
相关论文
共 19 条
[1]   INSULIN AND INSULIN-LIKE GROWTH FACTOR-I BINDING TO CULTURED RAT GLOMERULAR MESANGIAL CELLS [J].
ABRASS, CK ;
RAUGI, GJ ;
GABOUREL, LS ;
LOVETT, DH .
ENDOCRINOLOGY, 1988, 123 (05) :2432-2439
[2]   RECEPTORS FOR AND EFFECTS OF INSULIN AND IGF-I IN RAT GLOMERULAR MESANGIAL CELLS [J].
ARNQVIST, HJ ;
BALLERMANN, BJ ;
KING, GL .
AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 254 (03) :C411-C416
[3]  
BRENNER BM, 1981, ACTA ENDOCRINOL-COP, V97, P7
[4]  
CONTI F, 1988, DIABETOLOGIA, V31, pA481
[5]   STUDIES ON BINDING AND MITOGENIC EFFECT OF INSULIN AND INSULIN-LIKE GROWTH FACTOR-I IN GLOMERULAR MESANGIAL CELLS [J].
CONTI, FG ;
STRIKER, LJ ;
LESNIAK, MA ;
MACKAY, K ;
ROTH, J ;
STRIKER, GE .
ENDOCRINOLOGY, 1988, 122 (06) :2788-2795
[6]   TISSUE CONCENTRATIONS OF SOMATOMEDIN-C - FURTHER EVIDENCE FOR MULTIPLE SITES OF SYNTHESIS AND PARACRINE OR AUTOCRINE MECHANISMS OF ACTION [J].
DERCOLE, AJ ;
STILES, AD ;
UNDERWOOD, LE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (03) :935-939
[7]   RELATIVE INCREASE IN INSULIN-LIKE GROWTH FACTOR-I MESSENGER-RIBONUCLEIC-ACID LEVELS IN COMPENSATORY RENAL HYPERTROPHY [J].
FAGIN, JA ;
MELMED, S .
ENDOCRINOLOGY, 1987, 120 (02) :718-724
[8]  
FLYVBJERG A, 1988, DIABETOLOGIA, V31, P310
[9]   SOMATOSTATIN ANALOG ADMINISTRATION PREVENTS INCREASE IN KIDNEY SOMATOMEDIN-C AND INITIAL RENAL GROWTH IN DIABETIC AND UNINEPHRECTOMIZED RATS [J].
FLYVBJERG, A ;
FRYSTYK, J ;
THORLACIUSUSSING, O ;
ORSKOV, H .
DIABETOLOGIA, 1989, 32 (04) :261-265
[10]   SPECIFIC, HIGH-AFFINITY RECEPTORS FOR INSULIN-LIKE GROWTH FACTOR-II IN THE RAT-KIDNEY GLOMERULUS [J].
HASKELL, JF ;
PILLION, DJ ;
MEEZAN, E .
ENDOCRINOLOGY, 1988, 123 (02) :774-780