A DISTAL REGION ENHANCES THE PROLACTIN INDUCED PROMOTER ACTIVITY OF THE RABBIT ALPHA-S1-CASEIN GENE

被引：20

作者：

PIERRE, S ^{[1
]}

JOLIVET, G ^{[1
]}

DEVINOY, E ^{[1
]}

THERON, MC ^{[1
]}

MALIENOUNGASSA, R ^{[1
]}

PUISSANT, C ^{[1
]}

HOUDEBINE, LM ^{[1
]}

机构：

[1] INRA,UNITE DIFFERENCIAT CELLULAIRE,F-78352 JOUY EN JOSAS,FRANCE

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 87卷 / 1-3期

关键词：

ORGANOIDS; TRANSFECTION; TRANSIENT EXPRESSION; CHO CELLS; HC11; CELLS; CHLORAMPHENICOL ACETYL TRANSFERASE;

D O I：

10.1016/0303-7207(92)90243-Y

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Casein gene expression is induced in the rabbit mammary gland by prolactin (PRL). Alpha-s1-casein is the major casein secreted into milk. In order to define the position of the DNA sequences involved in the control of rabbit alpha-s1-casein gene regulation by PRL, chimeric genes were constructed between upstream regions of the rabbit alpha-s1-casein gene and the chloramphenicol acetyl transferase (CAT) reporter gene. A series of 5'-deleted fusion genes was obtained by nuclease digestion of the alpha-s1-casein gene upstream region. These gene constructs were transfected into rabbit primary mammary cells, or cotransfected in CHO cells with the plasmid coding for the rabbit mammary receptor (PRL-R). A regulatory region has been located between nt -3768 and -3155. This region enhances the prolactin induced promoter activity of the alpha-s1-casein gene. It might possess or cooperate with prolactin responsive elements located further downstream in the alpha-s1-casein gene.

引用

页码：147 / 156

页数：10

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