PURIFICATION AND CRYSTALLIZATION OF LACTOBACILLUS-CASEI FOLYLPOLYGLUTAMATE SYNTHETASE EXPRESSED IN ESCHERICHIA-COLI

被引：7

作者：

CODY, V ^{[1
]}

LUFT, JR ^{[1
]}

PANGBORN, W ^{[1
]}

TOY, J ^{[1
]}

BOGNAR, AL ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MICROBIOL,TORONTO M5S 1A8,ONTARIO,CANADA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 224卷 / 04期

关键词：

FOLYLPOLYGLUTAMATE SYNTHETASE; FOLATE; POLYGLUTAMATES; CRYSTALLIZATION;

D O I：

10.1016/0022-2836(92)90480-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Folylpolyglutamate synthetase (FPGS) from Lactobacillus casei has been crystallized with polyethylene glycol and acetate buffer at pH 5·0. The enzyme was obtained from Escherichia coli strain SF4 harboring the L. casei FPGS chromosomal gene on a pEMBL vector (pGT3-8·1). Crystals of the enzyme were obtained which diffract to 2·6 Å resolution. The crystals are monoclinic, space group P21, with unit cell dimensions of a = 54·07 A ̊, b = 45·83 A ̊, c = 84·37 A ̊ and β = 107·92 °. A unit cell contains one molecule of the 43,000 Da enzyme per asymmetric unit. A complete X-ray data set on the native crystals has been collected. © 1992.

引用

页码：1179 / 1180

页数：2

共 7 条

[1]

BOGNAR AL, 1983, J BIOL CHEM, V258, P2574

[2]

BOGNAR AL, 1987, J BIOL CHEM, V262, P12337

[3]

BOGNAR AL, 1985, J BIOL CHEM, V260, P5625

[4] MAMMALIAN FOLYLPOLY-GAMMA-GLUTAMATE SYNTHETASE .1. PURIFICATION AND GENERAL-PROPERTIES OF THE HOG LIVER-ENZYME [J].

CICHOWICZ, DJ ;

SHANE, B .

BIOCHEMISTRY, 1987, 26 (02) :504-512

[5] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].

JANCARIK, J ;

KIM, SH .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411

[6]

SHANE B, 1980, J BIOL CHEM, V255, P5655

[7]

TOY J, 1990, J BIOL CHEM, V265, P2492

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