CONSTRUCTION OF YEAST ARTIFICIAL CHROMOSOME (YAC) CLONE BANKS COVERING 3 GENOME EQUIVALENTS AND ISOLATION OF YACS CONTAINING THE HUMAN GENE ENCODING TUMOR-NECROSIS-FACTOR RECEPTOR-BETA

被引：4

作者：

DELOUKAS, P

AIGNER, L

KANIA, M

VANLOON, APGM

机构：

[1] F HOFFMANN LA ROCHE & CO LTD,VITAMIN RES BIOTECHNOL,GRENZACHERSTR 124,CH-4002 BASEL,SWITZERLAND

[2] F HOFFMANN LA ROCHE & CO LTD,PHARMACEUT RES DEPT,NEW TECHNOL,CH-4002 BASEL,SWITZERLAND

来源：

GENE | 1992年 / 110卷 / 02期

关键词：

RECOMBINANT DNA; GENE STRUCTURE; VECTOR COMPARISON; IMMUNOLOGY; EXONS; INTRONS;

D O I：

10.1016/0378-1119(92)90647-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Yeast artificial chromosome (YAC) banks covering in total about three haploid genome equivalents were constructed using a human Epstein-Barr-virus-transformed B lymphocytic cell line. Two clone banks were made: 20 000 clones with average inserts of 350 kb in the pYAC4 vector and 9850 clones with average inserts of 180 kb using vectors pJS89 and pJS91. Direct comparison of pYAC4 with pJS89 and pJS91 showed pYAC4 to be the most suitable cloning vector. Two partial banks with average insert sizes of 220 kb for human endothelial cell DNA and epithelial HEp2 cell DNA were also constructed, each covering 10% of the haploid genome. A rapid, three-step PCR screening procedure for isolation of individual YAC clones was developed and used to identify two clones encoding TNF-R-beta. These clones cover about 200 kb and have 170 kb in common. TNF-R-beta is 9.3 kb long and contains two introns within the protein-coding sequence.

引用

收藏

页码：189 / 195

页数：7

相关论文

共 28 条

[1] YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN XQ24-XQ28 DNA - LIBRARY CONSTRUCTION AND REPRESENTATION OF PROBE SEQUENCES [J].

ABIDI, FE ;

WADA, M ;

LITTLE, RD ;

SCHLESSINGER, D .

GENOMICS, 1990, 7 (03) :363-376

[2] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS [J].

ALBERTSEN, HM ;

ABDERRAHIM, H ;

CANN, HM ;

DAUSSET, J ;

LEPASLIER, D ;

COHEN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4256-4260

[3] A 3.5 GENOME EQUIVALENT MULTIACCESS YAC LIBRARY - CONSTRUCTION, CHARACTERIZATION, SCREENING AND STORAGE [J].

ANAND, R ;

RILEY, JH ;

BUTLER, R ;

SMITH, JC ;

MARKHAM, AF .

NUCLEIC ACIDS RESEARCH, 1990, 18 (08) :1951-1956

[4] IDENTIFICATION OF 2 TYPES OF TUMOR-NECROSIS-FACTOR RECEPTORS ON HUMAN CELL-LINES BY MONOCLONAL-ANTIBODIES [J].

BROCKHAUS, M ;

SCHOENFELD, HJ ;

SCHLAEGER, EJ ;

HUNZIKER, W ;

LESSLAUER, W ;

LOETSCHER, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :3127-3131

[5] TRANSFORMATION OF YEAST SPHEROPLASTS WITHOUT CELL-FUSION [J].

BURGERS, PMJ ;

PERCIVAL, KJ .

ANALYTICAL BIOCHEMISTRY, 1987, 163 (02) :391-397

[6] CLONING OF LARGE SEGMENTS OF EXOGENOUS DNA INTO YEAST BY MEANS OF ARTIFICIAL CHROMOSOME VECTORS [J].

BURKE, DT ;

CARLE, GF ;

OLSON, MV .

SCIENCE, 1987, 236 (4803) :806-812

[7] BASE SEQUENCE STUDIES OF 300 NUCLEOTIDE RENATURED REPEATED HUMAN DNA CLONES [J].

DEININGER, PL ;

JOLLY, DJ ;

RUBIN, CM ;

FRIEDMANN, T ;

SCHMID, CW .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 151 (01) :17-33

[8] STREAMLINED APPROACH TO CREATING YEAST ARTIFICIAL CHROMOSOME LIBRARIES FROM SPECIALIZED CELL SOURCES [J].

FEINGOLD, JM ;

OGDEN, SD ;

DENNY, CT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8637-8641

[9] MOLECULAR-CLONING AND EXPRESSION OF THE TYPE-1 AND TYPE-2 MURINE RECEPTORS FOR TUMOR-NECROSIS-FACTOR [J].

GOODWIN, RG ;

ANDERSON, D ;

JERZY, R ;

DAVIS, T ;

BRANNAN, CI ;

COPELAND, NG ;

JENKINS, NA ;

SMITH, CA .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (06) :3020-3026

[10] SYSTEMATIC SCREENING OF YEAST ARTIFICIAL-CHROMOSOME LIBRARIES BY USE OF THE POLYMERASE CHAIN-REACTION [J].

GREEN, ED ;

OLSON, MV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1213-1217