KINETICS OF ANTIBODY-BINDING AT SOLID-LIQUID INTERFACES IN FLOW

被引:38
作者
WEMHOFF, GA
RABBANY, SY
KUSTERBECK, AW
OGERT, RA
BREDEHORST, R
LIGLER, FS
机构
[1] USN,RES LAB,CTR BIOMOLEC SCI & ENGN,CODE 6090,WASHINGTON,DC 20375
[2] UNIV HAMBURG,DEPT BIOCHEM & MOLEC BIOL,W-2000 HAMBURG 13,GERMANY
[3] HOFSTRA UNIV,DEPT ENGN,BIOENGN PROGRAM,HEMPSTEAD,NY 11550
关键词
DISPLACEMENT; IMMUNOASSAY; BIOSENSOR;
D O I
10.1016/0022-1759(92)90029-S
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We have developed the theoretical framework for a displacement immunoassay conducted in flow under nonequilibrium conditions. Using a repetitive displacement technique, we determined the displacement rate and apparent dissociation rate constant at different flow rates. Our data suggest that the kinetics are best described by a first-order function. The displacement efficiency, the displacement rate, and therefore the apparent dissociation rate constant were calculated and demonstrated to be flow rate dependent. The theoretical framework developed in this study was successful in predicting the behavior of antigen displacement in flow.
引用
收藏
页码:223 / 230
页数:8
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