SENSITIVE AND STABLE COOKSON-TYPE REAGENT FOR DERIVATIZATION OF CONJUGATED DIENES FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：19

作者：

SHIMADA, K

MIZUGUCHI, T

机构：

[1] Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, 920

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 606卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)85266-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and stable Cookson-type reagent, 4-substituted 1,2,4-triazoline-3,5-dione, having 6-methoxy-2-phenylbenzoxazole as a fluorophore, was prepared for high-performance liquid chromatographic measurements of conjugated dienes. The reagent was purified by sublimation to give stable purple crystals. The reagent quantitatively produced the adduct with provitamin D3 in 5 min under ice cooling, which was highly responsive to fluorescence detection [detection limit 2 fmol per injection (signal-to-noise ratio = 5)].

引用

页码：133 / 135

页数：3

共 8 条

[1]

Cookson RC., 1971, ORG SYNTH, V51, P121, DOI [DOI 10.15227/0RGSYN.051.0121, 10.15227/orgsyn.051.0121, DOI 10.15227/ORGSYN.051.0121]

[2] DETERMINATION OF 25-HYDROXYVITAMIN-D3 IN HUMAN SERUM BY FLUORESCENCE LABELING AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].