JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS
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1993年
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32卷
/
6B期
关键词:
SCANNING TUNNELING MICROSCOPY;
ATOMIC FORCE MICROSCOPY;
T4-BACTERIOPHAGE;
TOBACCO MOSAIC VIRUS;
D O I:
10.1143/JJAP.32.2962
中图分类号:
O59 [应用物理学];
学科分类号:
摘要:
Bacteriophage T4 and tobacco mosaic virus were imaged by scanning tunneling microscopy after metal coating of the specimens and by atomic force microscopy without coating, and their topographies were examined. A metal coating approximately 5 nm in thickness assured easy and reproducible imaging of samples but obscured their detailed structural features. AFM gave a better resolution on this scale, revealing the presence of tail fibers 2 nm in diameter of T4 phage, which is the best resolution so far reported for isolated biological structures. Sample topographies were in good agreement with the results of previous electron microscopic analysis.