THE STAPHYLOCOCCUS-CARNOSUS SECE GENE - CLONING, NUCLEOTIDE-SEQUENCE, AND FUNCTIONAL-CHARACTERIZATION IN ESCHERICHIA-COLI SECE MUTANT STRAINS

被引：8

作者：

MEENS, J ^{[1
]}

KLOSE, M ^{[1
]}

FREUDL, R ^{[1
]}

机构：

[1] FORSCHUNGSZENTRUM JULICH, INST BIOTECHNOL 1, D-52425 JULICH, GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 117卷 / 01期

关键词：

PROTEIN SECRETION; SECE GENE; FUNCTIONAL COMPLEMENTATION; PREPROTEIN TRANSLOCASE; STAPHYLOCOCCUS-CARNOSUS;

D O I：

10.1016/0378-1097(94)90180-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A DNA fragment containing the genes secE, nusG and rplK of Staphylococcus carnsosus was cloned using the Escherichia coli rplK gene as a probe. The S. carnosus secE homologue encodes a protein of 65 amino acid residues which is homologous to the carboxyl-terminal region of the E. coli SecE protein. The S. carnosus SecE polypeptide which, in contrast to the E. coli SecE protein, contains only one putative transmembrane segment, could fully replace the E. coli SecE protein in two different secE mutants. These results strongly suggest that the identified secE gene encodes an important component of the S. carnosus protein export apparatus.

引用

页码：113 / 119

页数：7

共 25 条

[1]

BRUNDAGE L, 1992, J BIOL CHEM, V267, P4166

[2] THE SPC RIBOSOMAL-PROTEIN OPERON OF ESCHERICHIA-COLI - SEQUENCE AND COTRANSCRIPTION OF THE RIBOSOMAL-PROTEIN GENES AND A PROTEIN EXPORT GENE [J].

CERRETTI, DP ;

DEAN, D ;

DAVIS, GR ;

BEDWELL, DM ;

NOMURA, M .

NUCLEIC ACIDS RESEARCH, 1983, 11 (09) :2599-2616

[3]

Debabov V. G., 1982, MOL BIOL BACILLI, V1, P331

[4] SEQUENCE AND TRANSCRIPTIONAL PATTERN OF THE ESSENTIAL ESCHERICHIA-COLI SECE-NUSG OPERON [J].

DOWNING, WL ;

SULLIVAN, SL ;

GOTTESMAN, ME ;

DENNIS, PP .

JOURNAL OF BACTERIOLOGY, 1990, 172 (03) :1621-1627

[5] PROTEIN SECRETION IN GRAM-POSITIVE BACTERIA [J].

FREUDL, R .

JOURNAL OF BIOTECHNOLOGY, 1992, 23 (03) :231-240

[6]

FREUDL R, 1989, J MOL BIOL, V205, P711

[7] ISOLATION AND CHARACTERIZATION OF THE SECE HOMOLOG GENE OF BACILLUS-SUBTILIS [J].

JEONG, SM ;

YOSHIKAWA, H ;

TAKAHASHI, H .

MOLECULAR MICROBIOLOGY, 1993, 10 (01) :133-142

[8]

KLOSE M, 1993, J BIOL CHEM, V268, P4504

[9] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].

KYTE, J ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132

[10] SUPPRESSION OF GROWTH AND PROTEIN SECRETION DEFECTS IN ESCHERICHIA-COLI SECA MUTANTS BY DECREASING PROTEIN-SYNTHESIS [J].

LEE, CA ;

BECKWITH, J .

JOURNAL OF BACTERIOLOGY, 1986, 166 (03) :878-883

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