STOPPED-FLOW DETERMINATION OF DIPHACINONE BASED ON LANTHANIDE-SENSITIZED LUMINESCENCE

The stopped-flow mixing technique was used to investigate the application of kinetic methodology to complex formation reactions involving energy transfer processes. By using initial rate measurements a very rapid fluorimetric method was developed for the determination of diphacinone by formation of the ternary complex Eu(III)-diphacinone-ammonia in the presence of Triton X-100. Kinetic data can be obtained only 2-3 s after the reactants are mixed, which allows ready application of the method to routine analyses. The linear response range of the proposed method is 0.5-5 mug ml-1 diphacinone and the mid-range relative standard deviation is ca. 2%. The mean recovery obtained in the analysis of serum samples was 98.3%.