SEQUENCING AND GENE-EXPRESSION OF HYPODERMIN-A, HYPODERMIN-B, HYPODERMIN-C IN LARVAL STAGES OF HYPODERMA-LINEATUM

被引：32

作者：

MOIRE, N ^{[1
]}

BIGOT, Y ^{[1
]}

PERIQUET, G ^{[1
]}

BOULARD, C ^{[1
]}

机构：

[1] FAC SCI TOURS,INST BIOCENOT EXP AGROSYST,TOURS,FRANCE

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1994年 / 66卷 / 02期

关键词：

HYPODERMA LINEATUM; HYPODERMIN; SERINE PROTEASE;

D O I：

10.1016/0166-6851(94)90150-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNAs of hypodermins, enzymes secreted by the larvae of the parasitic fly Hypoderma lineatum, were sequenced. Four cDNA clones were isolated, one encoding hypodermin A (HA), one encoding hypodermin C (HC), and the two others encoding proteins related to hypodermin B (HB). The amino acid sequences deduced from the nucleotide sequences confirmed that these enzymes are serine proteases. HA and one of the HB proteins had potential N-linked glycosylation sites. Analysis of hypodermin protein, RNA and DNA at different larval stages indicated that protein overexpression is regulated transcriptionally for HA and HB, and by transcriptional and DNA amplification for HC.

引用

页码：233 / 240

页数：8

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