ANALYSIS OF THE IRON-SULFUR CLUSTERS WITHIN THE COMPLEX-I (NADH-UBIQUINONE OXIDOREDUCTASE) ISOLATED FROM POTATO-TUBER MITOCHONDRIA

被引：12

作者：

LIN, TI

SLED, VD

OHNISHI, T

BRENNICKE, A

GROHMANN, L

机构：

[1] INST GENBIOL FORSCH, D-14195 BERLIN, GERMANY

[2] UNIV ULM, W-7900 ULM, GERMANY

[3] UNIV PENN, DEPT BIOCHEM & BIOPHYS, JOHNSON RES FDN, PHILADELPHIA, PA 19104 USA

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 230卷 / 03期

关键词：

MITOCHONDRIA (PLANT); NADH-UBIQUINONE OXIDOREDUCTASE (COMPLEX I); EPR; IRON-SULFUR CLUSTER; SOLANUM TUBEROSUM;

D O I：

10.1111/j.1432-1033.1995.tb20652.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitochondrial complex I (NADH:ubiquinone oxidoreductase) isolated from potato (Solanum tuberosum) has been investigated for the presence of iron-sulfur clusters. EPR spectroscopic analysis detected signals arising from clusters N1, N2, N3 and N4. Quantitation of the content of iron and sulfur within the isolated complex I showed the preparation to contain 22.6 mol acid-labile sulfide and 30.4 mol iron/mol complex I. The iron-sulfur cluster composition of the plant complex I appears to be similar to the well-known composition found in Neurospora crassa.

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页码：1032 / 1036

页数：5

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