MICRO ESTIMATION OF RNA BY CUPRIC ION CATALYZED ORCINOL REACTION

被引：151

作者：

LIN, RIS

SCHJEIDE, OA

机构：

[1] Department of Biophysics, University of California at Los Angeles, Los Angeles

[2] Laboratory of Nuclear Medicine and Radiation Biology, University of California at Los Angeles, Los Angeles

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 27卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90061-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for estimation of purified RNA using cupric ion catalyzed orcinol has been studied and improved. If such an assay is carried out under the optimal conditions described here it would appear to be the most sensitive, accurate, and rapid method for routine RNA estimation currently available. The improved conditions include a combination of several factors such as replacement of the usual FeCl3 by a copper salt, increasing the concentration of orcinol in the reaction mixture, and increasing the heating time from the commonly used 20 minutes to 35 minutes. © 1969.

引用

页码：473 / &

共 31 条

[1] ALBAUM HG, 1947, J BIOL CHEM, V167, P369
[2] BARRENSCHEEN HK, 1942, Z PHYSL CHEM, V272, P81
[3] Diagnosis of pentosuria with the reagent discovered by me
Bial, M
[J]. DEUTSCHE MEDIZINISCHE WOCHENSCHRIFT, 1903, 29 : 477 - 478
[4] BIAL M, 1903, DEUT MED WOCHENSCHR, V29, P253
[5] CERIOTTI GIOVANNI, 1955, JOUR BIOL CHEM, V214, P59
[6] CHARGAFF E, 1955, NUCLEIC ACIDS, V1, P300
[7] COLOWICK SP, 1957, METHODS ENZYMOLOG ED, V3, P680
[8] Tissue nuclei acids 1. Ribonucleic acids and nucleotides in embryonic and adult tissue.
Davidson, JN
Waymouth, C
[J]. BIOCHEMICAL JOURNAL, 1944, 38 : 39 - 50
[9] DAVIDSON JN, 1964, PROGRESS NUCLEIC ED, V3, P1
[10] DAVIDSON JN, 1965, BIOCHEMISTRY NUCLEIC, P102

← 1 2 3 4 →