FLUORESCENCE STUDIES OF THE INTERACTION BETWEEN 1,N6-ETHENOADENOSINE MONOPHOSPHATE AND NUCLEOTIDES

AMP, GMP, TMP and CMP quench the fluorescence of 1,N6-ethenoadenosine monophosphate (ε{lunate}-AMP). The fluorescence spectrum of ε{lunate}-AMP-nucleotide system is identical with that of ε{lunate}-AMP itself, and the fluorescence decay kinetics follow a single-exponential decay law. The dependence of fluorescence yields and lifetimes upon the concentration of nucleotides shows that the fluorescence oI ε{lunate}-AMP is principally quenched in a dynamic process by AMP, TMP and CMP, while it is quenched in both dynamic and static processes by GMP. The quenching constants increase in the following order: GMP > AMP > TMP > CMP. © 1979.