THE EFFECT OF MICROBIAL-CONTAMINATION AND PH CHANGES IN STORAGE SOLUTIONS DURING INVITRO ASSAYS OF BONDING AGENTS

被引:11
作者
FOTOS, PG
DIAZARNOLD, AM
WILLIAMS, VD
机构
[1] Department of Oral Pathology, Diagnosis The University of Iowa, Iowa City
[2] Department of Family Dentistry College, Dentistry The University of Iowa, Iowa City
关键词
D O I
10.1016/0109-5641(90)90020-F
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
A study of pH changes in, and microbial colonization of, storage solutions being utilized during an in vitro evaluation of luting agent solubility has been made. Microbial growth in storage solutions was observed to occur at as high as 105 colony-forming units (cfu)/mL after seven days' incubation. A strong time-dependent inverse correlation between pH and cfu was found, with the pH dropping to as low as 5.3 (p<0.01). Decreases in pH were not found in presterilized control samples (p<0.05). Further, it was demonstrated that presterilization with ethylene oxide or the addition of respiratory inhibitors prevented both microbial colonization and the pH drop when compared with controls (p<0.001). These data suggest that studies of dental materials which include incubations in various fluids should be controlled for the potential effects of micro-organisms and their effect on pH. © 1990.
引用
收藏
页码:154 / 157
页数:4
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