RENAL TRANSPLANTATION IN INBRED RAT .8. QUANTITATION OF CELL PROLIFERATION DURING UNMODIFIED REJECTION

Interstitial cell proliferation has been quantitatcd during renal allograft rejection in five donor-recipient combinations by measuring the amount of incorporation of 123I-labelled 5-iodo-2’-deoxyuridine into newly synthesized deoxyribonucleic acid of the kidney.There is a proliferative peak across major histocompatibility barriers at day 5 with no statistically significant difference between the LBNF hybrid → Lewis and BN→ Lewis combination,while LBNF1→ L sens. LBN showed significantly moreincorporation of the tracer. In the case of locus identity, LF344F1→ L, the proliferative peak occurs during the 3rd week,and the kidney incorporation levels return to normal as the LF344F1→ L survives normally for an indefinite period of time.The method provides a means of quantitation of cell proliferation for whole organ systems and demonstrates that the amount of lymphoid mass accumulating during rejection is a function of immunogenetic disparity and preexisting sensitivity. © 1969 by The Williams and Wilkins Co.