CULTURE OF MAMMARY EPITHELIAL-CELLS FROM BOVINE-MILK

被引：27

作者：

BUEHRING, GC

机构：

[1] School of Public Health, University of California, Berkeley, Berkeley

来源：

JOURNAL OF DAIRY SCIENCE | 1990年 / 73卷 / 04期

关键词：

culture; mammary epithelium; milk;

D O I：

10.3168/jds.S0022-0302(90)78752-8

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Mammary epithelial cells are exfoliated into cows’ milk and comprise a component of the somatic cells enumerated for milk quality control. This paper described the finding that these epithelial cells can be recovered from milk and grown in culture. The cultured cells were characterized for growth rate and growth potential, morphology by light and electron microscopy, presence of esterases and cytokeratins, and sensitivity to storage at 4°C. Cultured mammary epithelial cells from milk may be useful to dairy scientists and mammary gland biologists. © 1990, American Dairy Science Association. All rights reserved.

引用

页码：956 / 963

页数：8

共 23 条

[1]

BESSIS M, 1973, LIVING BLOOD CELLS T, P292

[2]

BUEHRING GC, 1976, CANCER RES, V36, P3742

[3]

BUEHRING GC, 1972, J NATL CANCER I, V49, P1433

[4]

BUEHRING GC, 1989, PT21 P INT ASS BREAS

[5] MODULATION OF PHENOTYPE IN CULTURES OF HUMAN-MILK EPITHELIAL-CELLS AND ITS RELATION TO THE EXPRESSION OF A MEMBRANE ANTIGEN [J].

CHANG, SE ;

TAYLORPAPADIMITROU, J .

CELL DIFFERENTIATION, 1983, 12 (03) :143-154

[6]

COLLIER RJ, 1977, ENDOCRINOLOGY, V100, P1192

[7] CULTIVATION AND PROPERTIES OF BOVINE MAMMARY CELL CULTURES [J].

EBNER, KE ;

LARSON, BL ;

HAGEMAN, EC ;

HOOVER, CR .

EXPERIMENTAL CELL RESEARCH, 1961, 23 (02) :373-&

[8] JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIA [J].

FARQUHAR, MG ;

PALADE, GE .

JOURNAL OF CELL BIOLOGY, 1963, 17 (02) :375-&

[9] IDENTIFICATION AND CHARACTERIZATION OF EPITHELIAL-CELLS IN MAMMALIAN-TISSUES BY IMMUNOFLUORESCENCE MICROSCOPY USING ANTIBODIES TO PREKERATIN [J].

FRANKE, WW ;

APPELHANS, B ;

SCHMID, E ;

FREUDENSTEIN, C ;

OSBORN, M ;

WEBER, K .

DIFFERENTIATION, 1979, 15 (01) :7-25

[10]

Freshney RI, 1987, CULTURE ANIMAL CELLS, P57

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