3,3,3-TRIFLUORO-2-AMINOISOBUTYRATE - MECHANISM-BASED INHIBITOR OF PSEUDOMONAS-CEPACIA ALPHA-DIALKYLAMINO ACID TRANSAMINASE

被引：16

作者：

KELLER, JW

OLEARY, MH

机构：

[1] Department of Chemistry, University of Wisconsin, Madison

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)91149-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Racemic 3,3,3-trifluoro-2-aminoisobutyrate (F3AIB) causes complete, irreversible inactivation of Pseudomonas cepaciaα-dialkylamino acid transaminase. The inactivation reaction is first order in enzyme and is saturable with respect to F3AIB, with an apparent Michaelis constant of 0.15 mM. Competitive inhibition of the inactivation reaction by substrate, 2-aminoisobutyrate, and product, L-alanine, implicates complex formation between F3AIB and the enzyme active site. Tracer studies using [1-14C]F3AIB reveal that decarboxylation of F3AIB precedes inactivation. © 1979.

引用

页码：1104 / 1110

页数：7

共 10 条

[1] PURIFICATION AND PROPERTIES OF AN ALPHA-DIALKYL AMINO ACID TRANSAMINASE [J].