SIMULTANEOUS RECORDINGS OF GLUCOSE DEPENDENT ELECTRICAL-ACTIVITY AND ATP-REGULATED K+-CURRENTS IN ISOLATED MOUSE PANCREATIC BETA-CELLS

被引：169

作者：

SMITH, PA ^{[1
]}

ASHCROFT, FM ^{[1
]}

RORSMAN, P ^{[1
]}

机构：

[1] UNIV OXFORD,PHYSIOL LAB,PARKS RD,OXFORD OX1 3PT,ENGLAND

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

ATP-regulated K[!sup]+[!/sup]-channels; Glucose; Insulin secretion; Nystatin;

D O I：

10.1016/0014-5793(90)80667-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane potential and membrane currents were recorded from single mouse pancreatic β-cells using the perforated patch whole-cell recording technique at 30 °C. Single β-cells maintained in primary tissue culture exhibited glucose-dependent electrical activity similar to that reported for freshly isolated intact islets. The resting input conductance (5.1±0.9 nS) was determined by ATP-regulated K+ (KATP) channels as it was blocked by 1 mM tolbutamide. 8 mM glucose decreased the input conductance by 80%. The input conductance at -70 mV was of a similar value during the plateau phase and during the silent phase of electrical activity in 8 mM glucose. This suggests that oscillations of KATP channel activity do not underlie the slow waves. © 1990.

引用

页码：187 / 190

页数：4

共 9 条

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